Supporting Assays
Expanded In Vitro Tools to Characterize Drug Behavior
Complementary Assays to Deepen Insight into Pharmacokinetics.
As part of our comprehensive portfolio of ADME-Tox research services we provide the following supporting assays:
Cell Permeability
Cell Permeability assays allow early prediction of intestinal absorption, blood-brain barrier penetration, and tissue distribution.
Lysosomal Trapping
Lysosomal Trapping screening and mechanistic determination assays to predict propensity for lysosomal trapping. Lysosomal trapping is a physicochemical (non-enzymatic and non-transporter mediated) process by which membrane-bound organelles within hepatocytes can sequester lipophilic amine drugs, potentially limiting clearance and reducing therapeutic effect.
Plasma Protein Binding
Plasma Protein Binding determines free drug concentration (fraction unbound,) in plasma, which is required to inform dosage considerations for the clinic and is a necessary input for several PK-related calculations, modeling, and definitive in vitro study design.
Red Blood Cell Partitioning
Red Blood Cell Partitioning studies determine your drug’s blood to plasma ratio. Some drugs have a tendency to bind to red blood cells or become sequestered through passive diffusion into the cells. Evaluating potential drug sequestration in red blood cells in addition to plasma protein binding studies helps to prevent misinterpretation of pharmacokinetic data and the potential overestimation of a drug’s intrinsic clearance.
Individual Studies or As Part of Integrated Programs