Enzyme Induction
Enzyme Induction Studies to Evaluate Drug-Drug Interaction Potential
Enzyme induction studies predict if exposure to a test article will result in increased synthesis and concentration of liver enzymes that are important in drug disposition and pharmacokinetics.
Enzyme induction data is key to the evaluation of drug-drug interaction (DDI) potential of your compound.
Enzyme induction studies are critical for predicting pharmacokinetics because induction of these enzymes may result in increased clearance of the drug or another metabolized compound.
As part of our comprehensive portfolio of ADME-Tox research services we provide the following enzyme induction studies. Our study designs comply with current regulatory guidance enzyme for induction studies.
In Vitro CYP450 Induction
In Vitro CYP450 Induction studies evaluate test articles using primary cultures of hepatocytes from human and preclinical species.
Ex Vivo CYP & UGT Induction
Ex Vivo CYP450 Induction studies use microsomes isolated from excised livers of preclinical species dosed with test article to identify preclinical species with the closest induction profile to humans and are used to explain unexpected results in pharmacokinetic/pharmacodynamic (PK/PD), absorption, metabolism, distribution, and excretion (ADME), and toxicology studies.
Automation and Analysis
Enzyme induction studies are implemented in our state-of-the-art laboratory where we offer:
Validated qRT-PCR methods
Tecan liquid handling systems or manual incubations available
Validated LC-MS/MS methods for activity endpoints
Deuterated internal standards (all CYPs and most UGTs) for activity endpoints
Comprehensive reports