Antioxidant Assays
Evaluate supplements, cosmeceuticals and therapeutics
Free radicals and reactive oxygen species (ROS) are highly reactive molecules that are generated by normal cellular processes, environmental stresses, and UV irradiation. ROS react with cellular components and can damage DNA, carbohydrates, proteins, and lipids resulting in cellular and tissue injury. Excess production of ROS can also lead to inflammation, premature aging disorders, and may contribute to disease states, including cancer, diabetes, and atherosclerosis. Organisms have developed complex antioxidant systems to protect themselves from oxidative stress; however, excess ROS can overwhelm the systems and cause severe damage.
GainMed offers cell-based and enzyme-based screening assays for evaluating the total antioxidant activity of compounds and extracts. These assays can determine the total antioxidant capacity of biological fluids, cells, tissue, and naturally occurring or synthetic compounds for use as dietary supplements, topical protection, and therapeutics.
Cell-Based Antioxidant Assays
GainMed’s in vitro cell-based antioxidant assays evaluate compounds and extracts for their effect on modulating or mitigating cellular exposure to oxidative stress. GainMed’s cell-based antioxidant studies include:
Cellular Antioxidant Activity (CAA) Assays
Reactive Oxygen Species (ROS) Assays
Enzyme-Based Antioxidant Assays
GainMed’s enzyme-based antioxidant assays evaluate antioxidant enzyme activity in the presence of test compounds or extracts. GainMed’s enzyme-based antioxidant studies include:
Cellular Antioxidant Activity (CAA) Assays
- ABTS or TEAC (Trolox Equivalent Antioxidant Capacity) Assays
- ORAC Antioxidant Assay
- HORAC Antioxidant Assay
- NORAC Antioxidant Assay
- CLORAC Antioxidant Assay
- SOAC Antioxidant Assay
- DPPH Antioxidant Assay
- Cu-TAC Antioxidant Assay
- TAC-BCS Antioxidant Assay
- FRAP Assay
- Ferrous Iron Chelating (FIC) Assay
- Cupric Ion Chelating (CIC) Assay
- Total Phenolic Content Assay
- TBARS Assay
Tell Us About Your Study
Select from any of our studies and get in touch with one of our contract screening study specialists to find out how our medium-throughput designs can help you select the right compound to move forward.